Human Aquaporin 9 (AQP9) ELISA Kit | AQP9 elisa kit

Human Aquaporin 9 (AQP9) ELISA Kit

Cat. Num. AAA450619

• Gene Names: AQP9; SSC1; AQP-9; T17287; HsT17287
• Reactivity: Human
• Synonyms: Aquaporin 9 (AQP9); Human Aquaporin 9 (AQP9) ELISA Kit; SSC1; Aquaglyceroporin-9; Small solute channel 1; AQP9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AQP9. No significant cross-reactivity or interference between AQP9 and analogues was observed.
• Sequence Length: 295

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AQP9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AQP9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP9 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AQP9 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AQP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AQP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AQP9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157266307
• NCBI GeneID: 366
• NCBI Accession #: NP_066190.2
• NCBI GenBank Nucleotide #: NM_020980.4
• UniProt Accession #: O43315; Q9NP32
• Molecular Weight: 31,431 Da
• NCBI Official Full Name: aquaporin-9 isoform 1
• NCBI Official Synonym Full Names: aquaporin 9
• NCBI Official Symbol: AQP9
• NCBI Official Synonym Symbols: SSC1; AQP-9; T17287; HsT17287
• NCBI Protein Information: aquaporin-9
• UniProt Protein Name: Aquaporin-9
• Protein Family: Aquaporin
• UniProt Gene Name: AQP9
• UniProt Synonym Gene Names: SSC1; AQP-9

NCBI Description

The aquaporins are a family of water-selective membrane channels. This gene encodes a member of a subset of aquaporins called the aquaglyceroporins. This protein allows passage of a broad range of noncharged solutes and also stimulates urea transport and osmotic water permeability. This protein may also facilitate the uptake of glycerol in hepatic tissue . The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na+, K+, Cl-, and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol.