Human Aquaporin-9 ELISA Kit | AQP9 elisa kit

Human Aquaporin-9 ELISA Kit

Cat. Num. AAA760373

• Gene Names: AQP9; SSC1; AQP-9; HsT17287
• Reactivity: Human
• Synonyms: Aquaporin-9; Human Aquaporin-9 ELISA Kit; AQP9 (Aquaporin-9)/Aquaglyceroporin-9/Small solute channel 1; AQP9 elisa kit

• Reactivity: Human
• Sequence Length: 295

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AQP9 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human AQP9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human AQP9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human AQP9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman AQP9 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157266307
• NCBI GeneID: 366
• NCBI Accession #: NP_066190.2
• NCBI GenBank Nucleotide #: NM_020980.3
• Molecular Weight: 31,431 Da
• NCBI Official Full Name: aquaporin-9
• NCBI Official Synonym Full Names: aquaporin 9
• NCBI Official Symbol: AQP9
• NCBI Official Synonym Symbols: SSC1; AQP-9; HsT17287
• NCBI Protein Information: aquaporin-9
• UniProt Protein Name: Aquaporin-9
• Protein Family: Aquaporin
• UniProt Gene Name: AQP9
• UniProt Synonym Gene Names: SSC1; AQP-9
• UniProt Entry Name: AQP9_HUMAN

NCBI Description

The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, multi-pass; Transporter; Channel, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q

Cellular Component: basolateral plasma membrane; integral to membrane; integral to plasma membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: amine transmembrane transporter activity; carboxylic acid transmembrane transporter activity; glycerol channel activity; polyol transmembrane transporter activity; porin activity; purine transmembrane transporter activity; pyrimidine transmembrane transporter activity; urea transmembrane transporter activity; water channel activity; water transporter activity

Biological Process: amine transport; canalicular bile acid transport; carboxylic acid transport; excretion; glycerol transport; immune response; metabolic process; polyol transport; purine transport; pyrimidine transport; response to mercury ion; response to organic substance; response to osmotic stress; transmembrane transport; transport; water homeostasis; water transport