Human Aquaporin 9 (AQP9) ELISA Kit | AQP9 elisa kit

Human Aquaporin 9 (AQP9) ELISA Kit

Cat. Num. AAA2020686

• Gene Names: AQP9; SSC1; AQP-9; HsT17287
• Reactivity: Human
• Synonyms: Aquaporin 9 (AQP9); Human Aquaporin 9 (AQP9) ELISA Kit; SSC1; Aquaglyceroporin-9; Small solute channel 1; AQP9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aquaporin 9 (AQP9).; No significant cross-reactivity or interference between Aquaporin 9 (AQP9) and analogues was observed.
• Sequence Length: 24

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 6.4pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 9 (AQP9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 9 (AQP9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 9 (AQP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 9 (AQP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 9 (AQP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aquaporin 9 (AQP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AQP9 elisa kit

Metabolic pathway; Infection immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 6759671
• NCBI GeneID: 366
• UniProt Accession #: O43315; Q9NP32
• Molecular Weight: 31,431 Da
• NCBI Official Full Name: aquaporin 9, partial
• NCBI Official Synonym Full Names: aquaporin 9
• NCBI Official Symbol: AQP9
• NCBI Official Synonym Symbols: SSC1; AQP-9; HsT17287
• NCBI Protein Information: aquaporin-9
• UniProt Protein Name: Aquaporin-9
• Protein Family: Aquaporin
• UniProt Gene Name: AQP9
• UniProt Synonym Gene Names: SSC1; AQP-9
• UniProt Entry Name: AQP9_HUMAN

NCBI Description

The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Channel, misc.; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; basolateral plasma membrane; plasma membrane; integral to membrane

Molecular Function: purine transmembrane transporter activity; water transporter activity; pyrimidine transmembrane transporter activity; amine transmembrane transporter activity; urea transmembrane transporter activity; carboxylic acid transmembrane transporter activity; polyol transmembrane transporter activity; water channel activity; porin activity; glycerol channel activity

Biological Process: polyol transport; glycerol transport; cellular water homeostasis; metabolic process; amine transport; water transport; carboxylic acid transport; response to osmotic stress; excretion; purine transport; response to mercury ion; response to organic substance; water homeostasis; pyrimidine transport; transport; canalicular bile acid transport; immune response; transmembrane transport