Typical Testing Data/Standard Curve (for reference only)
Rat Aquaporin 9 ELISA Kit | AQP-9 elisa kit
Rat Aquaporin 9 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AQP-9 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AQP-9. Standards or samples are then added to the microtiter plate wells and AQP-9 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AQP-9 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AQP-9 are added to each well to “sandwich” the AQP-9 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AQP-9 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-9 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. [provided by RefSeq, Jul 2008]
Uniprot Description
AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Transporter; Channel, misc.; Membrane protein, multi-pass; Transporter, aquaporin family; Membrane protein, integral
Chromosomal Location of Human Ortholog: 15q
Cellular Component: intracellular membrane-bound organelle; basolateral plasma membrane; integral to plasma membrane; integral to membrane; plasma membrane
Molecular Function: purine transmembrane transporter activity; water transporter activity; pyrimidine transmembrane transporter activity; amine transmembrane transporter activity; urea transmembrane transporter activity; polyol transmembrane transporter activity; carboxylic acid transmembrane transporter activity; water channel activity; porin activity; glycerol channel activity
Biological Process: polyol transport; glycerol transport; cellular water homeostasis; metabolic process; water transport; amine transport; carboxylic acid transport; response to osmotic stress; excretion; purine transport; water homeostasis; response to organic substance; response to mercury ion; transport; pyrimidine transport; canalicular bile acid transport; immune response; transmembrane transport
Research Articles on AQP-9
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Product Notes
The Rat AQP-9 aqp9 (Catalog #AAA728355) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728355 ELISA Kit recognizes Rat AQP-9. It is sometimes possible for the material contained within the vial of "Aquaporin 9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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