Goat Aquaporin 9 ELISA Kit | AQP-9 elisa kit

Goat Aquaporin 9 ELISA Kit

Cat. Num. AAA737161

• Gene Names: AQP9; SSC1; AQP-9; HsT17287
• Reactivity: Goat
• Synonyms: Aquaporin 9; Goat Aquaporin 9 ELISA Kit; AQP-9 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This AQP-9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat AQP-9 This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-9 elisa kit

Principle of the assay: AQP-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AQP-9 antibody and an AQP-9-HRP conjugate. The assay sample and buffer are incubated together with AQP-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP-9 concentration since AQP-9 from samples and AQP-9-HRP conjugate compete for the anti-AQP-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP-9 from the sample, fewer sites are left to bind AQP-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-9 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AQP-9 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 157266307
• NCBI GeneID: 366
• NCBI Accession #: NP_066190.2
• NCBI GenBank Nucleotide #: NM_020980.3
• Molecular Weight: 31,431 Da
• NCBI Official Full Name: aquaporin-9
• NCBI Official Synonym Full Names: aquaporin 9
• NCBI Official Symbol: AQP9
• NCBI Official Synonym Symbols: SSC1; AQP-9; HsT17287
• NCBI Protein Information: aquaporin-9; aquaglyceroporin-9; small solute channel 1
• UniProt Protein Name: Aquaporin-9
• UniProt Gene Name: AQP9
• UniProt Synonym Gene Names: SSC1; AQP-9
• UniProt Entry Name: AQP9_HUMAN

NCBI Description

The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Transporter; Membrane protein, multi-pass; Channel, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: purine transmembrane transporter activity; water transporter activity; amine transmembrane transporter activity; pyrimidine transmembrane transporter activity; urea transmembrane transporter activity; carboxylic acid transmembrane transporter activity; polyol transmembrane transporter activity; water channel activity; porin activity; glycerol channel activity

Biological Process: polyol transport; glycerol transport; cellular water homeostasis; metabolic process; water transport; amine transport; carboxylic acid transport; response to osmotic stress; excretion; purine transport; response to mercury ion; water homeostasis; response to organic substance; pyrimidine transport; transport; canalicular bile acid transport; immune response; transmembrane transport