Human ADAM22 ELISA Kit | ADAM22 elisa kit

Human ADAM22 (A Disintegrin And Metalloprotease 22) ELISA Kit

Cat. Num. AAA2516324

• Gene Names: ADAM22; MDC2; ADAM 22
• Reactivity: Human
• Synonyms: ADAM22; Human ADAM22 (A Disintegrin And Metalloprotease 22) ELISA Kit; ADAM22 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanADAM22. No significant cross-reactivity or interference between HumanADAM22 and analogues was observed.
• Sequence Length: 342

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM22 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanADAM22concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADAM22. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ADAM22and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADAM22, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ADAM22.You can calculate the concentration of ADAM22in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62204768
• NCBI GeneID: 53616
• Molecular Weight: 96,637 Da
• NCBI Official Full Name: ADAM22 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 22
• NCBI Official Symbol: ADAM22
• NCBI Official Synonym Symbols: MDC2; ADAM 22
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 22; a disintegrin and metalloproteinase domain 22; metalloproteinase-disintegrin ADAM22-3; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 22
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM22
• UniProt Synonym Gene Names: MDC2; ADAM 22
• UniProt Entry Name: ADA22_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]

Uniprot Description

ADAM22: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: axon; integral to membrane

Molecular Function: integrin binding; protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: central nervous system development; adult locomotory behavior; negative regulation of cell adhesion; proteolysis; cell adhesion; myelination in the peripheral nervous system