Human A Disintegrin And Metalloprotease 22 (ADAM22) ELISA Kit | ADAM22 elisa kit

Human A Disintegrin And Metalloprotease 22 (ADAM22) ELISA Kit

Cat. Num. AAA2023083

• Gene Names: ADAM22; MDC2; ADAM 22
• Reactivity: Human
• Synonyms: A Disintegrin And Metalloprotease 22 (ADAM22); Human A Disintegrin And Metalloprotease 22 (ADAM22) ELISA Kit; MDC2; Disintegrin And Metalloproteinase Domain-Containing Protein 22; Metalloproteinase-Like; Disintegrin-Like; And Cysteine-Rich Protein 2; ADAM22 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 22 (ADAM22).; No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 22 (ADAM22) and analogues was observed.
• Sequence Length: 870

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 22 (ADAM22) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 22 (ADAM22) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM22 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 22 (ADAM22). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 22 (ADAM22). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 22 (ADAM22), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 22 (ADAM22) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ADAM22 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 62204768
• NCBI GeneID: 53616
• UniProt Accession #: Q9P0K1; O75075; O75076; Q9P0K2; Q9UIA1; Q9UKK2
• Molecular Weight: 96,637 Da
• NCBI Official Full Name: ADAM22 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 22
• NCBI Official Symbol: ADAM22
• NCBI Official Synonym Symbols: MDC2; ADAM 22
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 22
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 22
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM22
• UniProt Synonym Gene Names: MDC2; ADAM 22
• UniProt Entry Name: ADA22_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]

Uniprot Description

ADAM22: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: axon; integral to membrane

Molecular Function: integrin binding; protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: central nervous system development; adult locomotory behavior; negative regulation of cell adhesion; cell adhesion; proteolysis; myelination in the peripheral nervous system