A Disintegrin And Metalloprotease 22 Recombinant Protein | ADAM22 recombinant protein

Recombinant A Disintegrin And Metalloprotease 22 (ADAM22)

Cat. Num. AAA2033170

• Gene Names: ADAM22; MDC2; ADAM 22
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: A Disintegrin And Metalloprotease 22; Recombinant A Disintegrin And Metalloprotease 22 (ADAM22); MDC2; Disintegrin And Metalloproteinase Domain-Containing Protein 22; Metalloproteinase-Like; Disintegrin-Like; And Cysteine-Rich Protein 2; ADAM22 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: L AQSLAHNIGI ISDKRKLASG ECKCEDTWSG CIMGDTGYYL PKKFTQCNIE EYHDFLNSGG GACLFNKPSK LLDPPECGNG FIETGEECDC GTPAECVLEG AECCKKCTLT QDSQCSDGLC CKKCKFQPMG TVCREAVNDC DIRETCSGNS SQCAPNIHKM DGYSCDGVQGICFGGRCKTR DRQCKYIWGQ KVTASDKYCY EKLNIEGTEK GN
• Sequence Length: 342

• Applicable Applications for ADAM22 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 27.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Leu370~Asn582
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 62204768
• NCBI GeneID: 53616
• Molecular Weight: 96,637 Da
• NCBI Official Full Name: ADAM22 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 22
• NCBI Official Symbol: ADAM22
• NCBI Official Synonym Symbols: MDC2; ADAM 22
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 22
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 22
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM22
• UniProt Synonym Gene Names: MDC2; ADAM 22
• UniProt Entry Name: ADA22_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants.[provided by RefSeq, Dec 2010]

Uniprot Description

ADAM22: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: axon; integral to membrane; plasma membrane

Molecular Function: integrin binding; metalloendopeptidase activity; protein binding; zinc ion binding

Biological Process: adult locomotory behavior; cell adhesion; central nervous system development; myelination in the peripheral nervous system; negative regulation of cell adhesion; proteolysis