Western Blot (WB)
(Western blot analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Rabbit TEM1 Polyclonal Antibody | anti-CD248 antibody
TEM1 Antibody
Gene Names
CD248; TEM1; CD164L1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
TEM1 Antibody is affinity chromatography purified via peptide column.
Synonyms
TEM1; Polyclonal Antibody; TEM1 Antibody; CD164L1; Endosialin; Tumor endothelial marker 1; CD248 molecule; endosialin; anti-CD248 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.
Purity/Purification
TEM1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
757
Applicable Applications for anti-CD248 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
TEM1 antibody can be used for detection of TEM1 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
TEM1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human TEM1.
Buffer
TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TEM1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of TEM1 in human colon tissue with TEM1 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of TEM1 in human colon tissue with TEM1 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of TEM1 in Human Colon tissue with TEM1 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of TEM1 in Human Colon tissue with TEM1 antibody at 20 μg/mL.)
Related Product Information for anti-CD248 antibody
TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,241 Da
NCBI Official Full Name
endosialin
NCBI Official Synonym Full Names
CD248 molecule, endosialin
NCBI Official Symbol
CD248
NCBI Official Synonym Symbols
TEM1; CD164L1
NCBI Protein Information
endosialin; 2610111G01Rik; CD164 sialomucin-like 1; CD248 antigen, endosialin; tumor endothelial marker 1
UniProt Protein Name
Endosialin
Protein Family
UniProt Gene Name
CD248
UniProt Synonym Gene Names
CD164L1; TEM1
UniProt Entry Name
CD248_HUMAN
Uniprot Description
CD248: May play a role in tumor angiogenesis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: proteinaceous extracellular matrix; cytoplasm; integral to membrane
Molecular Function: extracellular matrix binding; calcium ion binding; carbohydrate binding
Biological Process: cell migration; positive regulation of cell proliferation; lymph node development; anatomical structure regression
Research Articles on CD248
Similar Products
Product Notes
The CD248 cd248 (Catalog #AAA151280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TEM1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TEM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). TEM1 antibody can be used for detection of TEM1 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CD248 cd248 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TEM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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