Testing Data
Human A2M/Alpha2-Macroglobulin ELISA Kit | A2M elisa kit
Human A2M/Alpha2-Macroglobulin Quick ELISA Kit
O.D.|0.067|0.190|0.267|0.419|0.709|1.285|2.017|2.532
n|16|16|16|
Mean(pg/ml)|1336|3774|26412|
Standard deviation|93.52|233.99|1980.9|
CV(%)|7%|6.2%|7.5%|
n|24|24|24
Mean(pg/ml)|1412|3693|26457
Standard deviation|108.72|249.67|2090.1
CV(%)|7.7%|6.3%|7.9%
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 1336|1254|1452|1396|1360|61.18|4.5%
Sample 2: 3774|3674|3364|3354|3542|254.99|7.2%
Sample 3: 26412|33012|29451|29456|29583|1804.55|6.1%
*number of samples for each test n=16.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
To measure Human A2M, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human A2M in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human A2M in the sample.
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]
Uniprot Description
A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.
Protein type: Secreted, signal peptide; Inhibitor; Secreted
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: extracellular region; cytosol
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-1 binding; interleukin-8 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding
Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; regulation of small GTPase mediated signal transduction; extracellular matrix organization and biogenesis; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway
Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency
Research Articles on A2M
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Product Notes
The Human A2M a2m (Catalog #AAA1753947) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1753947 ELISA Kit recognizes Human A2M. It is sometimes possible for the material contained within the vial of "A2M/Alpha2-Macroglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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