Human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) DIY ELISA Kit | WNT3A diy elisa kit

Human Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit DIY Materials

Cat. Num. AAA2089434

• Reactivity: Human
• Synonyms: Wingless Type MMTV Integration Site Family; Member 3A (WNT3A); Human Wingless Type MMTV Integration Site Family; Member 3A (WNT3A) ELISA Kit DIY Materials; WNT3A diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3A (WNT3A). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) and analogues was observed.
• Sequence Length: 352

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for WNT3A diy elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119590235
• NCBI GeneID: 89780
• Molecular Weight: 42,905 Da
• NCBI Official Full Name: wingless-type MMTV integration site family, member 3A
• NCBI Official Synonym Full Names: Wnt family member 3A
• NCBI Official Symbol: WNT3A
• NCBI Protein Information: protein Wnt-3a
• UniProt Protein Name: Protein Wnt-3a
• UniProt Gene Name: WNT3A

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane

Molecular Function: frizzled binding; protein binding; receptor agonist activity; receptor binding; transcription coactivator activity

Biological Process: cell proliferation in forebrain; cell proliferation in midbrain; negative regulation of neurogenesis; neuron differentiation; palate development; positive regulation of cell proliferation; positive regulation of mesodermal cell fate specification; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; signalosome assembly; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin