Human Collagen Type IV (COL4) DIY ELISA Kit | COL4 diy elisa kit

Human Collagen Type IV (COL4) ELISA Kit DIY Materials

Cat. Num. AAA2088469

• Gene Names: COL4A5; ATS; ASLN; CA54
• Reactivity: Human
• Synonyms: Collagen Type IV (COL4); Human Collagen Type IV (COL4) ELISA Kit DIY Materials; Type-IV Collagen; COL4 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Collagen Type IV (COL4). No significant cross-reactivity or interference between Collagen Type IV (COL4) and analogues was observed.
• Sequence Length: 18

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 7.8-500ng/mL
• Sensitivity: 3.1ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for COL4 diy elisa kit

Tumor immunity; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 4261609
• NCBI GeneID: 1287
• Molecular Weight: 161,632 Da
• NCBI Official Full Name: collagen type IV alpha 5 chain, partial
• NCBI Official Synonym Full Names: collagen type IV alpha 5 chain
• NCBI Official Symbol: COL4A5
• NCBI Official Synonym Symbols: ATS; ASLN; CA54
• NCBI Protein Information: collagen alpha-5(IV) chain
• UniProt Protein Name: Collagen alpha-5(IV) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL4A5

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: endoplasmic reticulum lumen; extracellular region

Biological Process: collagen catabolic process; extracellular matrix organization and biogenesis

Disease: Alport Syndrome, X-linked