Human Collagen Type IV (COL4) ELISA Kit | COL4 elisa kit

Human Collagen Type IV (COL4) ELISA Kit

Cat. Num. AAA2022979

• Gene Names: COL4A5; ATS; ASLN; CA54
• Reactivity: Human
• Synonyms: Collagen Type IV (COL4); Human Collagen Type IV (COL4) ELISA Kit; Type-IV Collagen; COL4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL4. No significant cross-reactivity or interference between COL4 and analogues was observed.
• Sequence Length: 1,691

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8-500ng/mL
• Sensitivity: < 3.1ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for COL4 elisa kit

Tumor immunity; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 4261609
• NCBI GeneID: 1287
• Molecular Weight: 161,632 Da
• NCBI Official Full Name: collagen type IV alpha 5 chain, partial
• NCBI Official Synonym Full Names: collagen, type IV, alpha 5
• NCBI Official Symbol: COL4A5
• NCBI Official Synonym Symbols: ATS; ASLN; CA54
• NCBI Protein Information: collagen alpha-5(IV) chain
• UniProt Protein Name: Collagen alpha-5(IV) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL4A5
• UniProt Entry Name: CO4A5_HUMAN

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction

Molecular Function: extracellular matrix structural constituent

Biological Process: extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; neuromuscular junction development

Disease: Alport Syndrome, X-linked