Human Collagen Type IV (COL4) ELISA Kit | COL4 elisa kit

Human Collagen Type IV (COL4) ELISA Kit

Cat. Num. AAA2701454

• Gene Names: COL4A5; ATS; ASLN; CA54
• Reactivity: Human
• Synonyms: Collagen Type IV (COL4); Human Collagen Type IV (COL4) ELISA Kit; Type-IV Collagen; COL4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL4. No significant cross-reactivity or interference between COL4 and analogues was observed.
• Sequence Length: 18

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8-500ng/mL
• Sensitivity: < 3.3ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level COL4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level COL4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL4 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to COL4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of COL4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4261609
• NCBI GeneID: 1287
• Molecular Weight: 161,632 Da
• NCBI Official Full Name: collagen type IV alpha 5 chain, partial
• NCBI Official Synonym Full Names: collagen type IV alpha 5 chain
• NCBI Official Symbol: COL4A5
• NCBI Official Synonym Symbols: ATS; ASLN; CA54
• NCBI Protein Information: collagen alpha-5(IV) chain
• UniProt Protein Name: Collagen alpha-5(IV) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL4A5

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.