Rat Collagen Type IV (COL4) ELISA Kit | COL4 elisa kit

Rat Collagen Type IV (COL4) Elisa Kit

Cat. Num. AAA268843

• Gene Names: COL4A5; ATS; ASLN; CA54
• Reactivity: Rat
• Synonyms: Collagen Type IV (COL4); Rat Collagen Type IV (COL4) Elisa Kit; COL4 elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 18

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/ml-0.156 ng/ml
• Sensitivity: Up to 0.05 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat COL4 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4261609
• NCBI GeneID: 1287
• Molecular Weight: 161,632 Da
• NCBI Official Full Name: collagen type IV alpha 5 chain, partial
• NCBI Official Synonym Full Names: collagen, type IV, alpha 5
• NCBI Official Symbol: COL4A5
• NCBI Official Synonym Symbols: ATS; ASLN; CA54
• NCBI Protein Information: collagen alpha-5(IV) chain; collagen alpha-5(IV) chain; dA24A23.1; dA149D17.3; collagen IV, alpha-5 polypeptide; collagen of basement membrane, alpha-5 chain
• UniProt Protein Name: Collagen alpha-5(IV) chain
• Protein Family: Zinc finger protein CONSTANS
• UniProt Gene Name: COL4A5
• UniProt Entry Name: CO4A5_HUMAN

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction

Molecular Function: extracellular matrix structural constituent

Biological Process: axon guidance; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; neuromuscular junction development

Disease: Alport Syndrome, X-linked