Typical Testing Data/Standard Curve (for reference only)
Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit | UBE3A clia kit
Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
Gene Names
Ube3a; As; AS; CG6190; das; DmelCG6190; dube3a; dube3A; dUBE3A; Dube3a; ube3a; UBE3A
Reactivity
Human
Synonyms
UBE3A (Ubiquitin Protein Ligase E3A); Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit; UBE3A clia kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinant UBE3A. No significant cross-reactivity or interference between UBE3A and analogues was observed.
Sequence Length
973
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for UBE3A clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of UBE3A concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to UBE3A. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UBE3A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UBE3A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of UBE3A. You can calculate the concentration of UBE3A in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to UBE3A. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UBE3A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UBE3A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of UBE3A. You can calculate the concentration of UBE3A in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,590 Da
NCBI Official Full Name
ubiquitin protein ligase E3A
NCBI Official Synonym Full Names
Ubiquitin protein ligase E3A
NCBI Official Symbol
Ube3a
NCBI Official Synonym Symbols
As; AS; CG6190; das; DmelCG6190; dube3a; dube3A; dUBE3A; Dube3a; ube3a; UBE3A
NCBI Protein Information
CG6190 gene product from transcript CG6190-RA
UniProt Protein Name
LD21888p
Protein Family
UniProt Gene Name
Ube3a
UniProt Entry Name
Q9VTH1_DROME
Research Articles on UBE3A
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Product Notes
The Human UBE3A ube3a (Catalog #AAA2530651) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2530651 CLIA Kit recognizes Human UBE3A. It is sometimes possible for the material contained within the vial of "UBE3A (Ubiquitin Protein Ligase E3A), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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