Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) CLIA Kit | LpPLA2 clia kit
Human LpPLA2 (Phospholipase A2, Lipoprotein Associated) CLIA Kit
Reactivity
Human
Synonyms
LpPLA2 (Phospholipase A2; Lipoprotein Associated); Human LpPLA2 (Phospholipase A2; Lipoprotein Associated) CLIA Kit; LpPLA2 clia kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinant LpPLA2. No significant cross-reactivity or interference between LpPLA2 and analogues was observed.
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LpPLA2 clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of LpPLA2 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to LpPLA2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LpPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LpPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of LpPLA2. You can calculate the concentration of LpPLA2 in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to LpPLA2. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LpPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LpPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of LpPLA2. You can calculate the concentration of LpPLA2 in the samples by comparing the RLU value of the samples to the standard curve.
