ICOS / AILIM / CD278 active protein

Recombinant Human ICOS / AILIM / CD278 Protein (His & Fc tag)

Cat. Num. AAA2546316

• Gene Names: ICOS; AILIM; CD278; CVID1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ICOS / AILIM / CD278; Recombinant Human ICOS / AILIM / CD278 Protein (His & Fc tag); AILIM; CD278; CVID1; ICOS / AILIM / CD278 active protein

• Host: Human Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20 mM Tris, 150mM NaCI, pH 8.5, 5% Trehalose 5% mannitol, 0.01% Tween-80
• Sequence Length: 199

• Application Notes: The recombinant human ICOS/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 368 amino acids and predicts a molecular mass of 41.6 kDa. The apparent molecular mass of rhICOS/Fc monomer is approximately 50 kDa due to glycosylation.
• Source: Human
• Predicted N Terminal: Glu 21
• Reconstitution: It is recommended that sterile water (400 uL) be added to the vial to prepare a stock solution of 0.25 mg/mL/.; Concentration is measured by UV-Vis.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Bio-Activity: Measured by its binding ability in a functional ELISA. Inmobilized human B7-H2 at 1 ug/mL/ (100 uL/ well.) can bind human ICOS with a linear range of 1.6-200- ng/mL.
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for ICOS / AILIM / CD278 active protein

Background: Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T-B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS is more potent in the induction of IL-10 production, a cytokine important for suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provides key second signals that can regulate the activation, inhibition and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested of being involved in the development of airway inflammation and airway hyperresponsiveness.

Description: A DNA sequence encoding the extracellular domain of human ICOS (NP_036224.1) (Met 1-Phe 141) was fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 15029518
• NCBI GeneID: 29851
• NCBI Accession #: NP_036224.1
• NCBI GenBank Nucleotide #: NM_012092.3
• UniProt Accession #: Q9Y6W8; Q8N6W8
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T-cell costimulator
• NCBI Official Symbol: ICOS
• NCBI Official Synonym Symbols: AILIM; CD278; CVID1
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• UniProt Gene Name: ICOS
• UniProt Synonym Gene Names: AILIM

NCBI Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33.2

Cellular Component: plasma membrane

Biological Process: T cell costimulation

Disease: Immunodeficiency, Common Variable, 1; Immunodeficiency, Common Variable, 2