ICOS recombinant protein

ICOS, 21-140aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206820

• Gene Names: ICOS; AILIM; CD278; CVID1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ICOS; 21-140aa; Human; hIgG-His-tag; Baculovirus; Inducible T-cell costimulator; AILIM; CD278; CVID1; ICOS recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM MES buffer (pH 5.5) containing 40% glycerol, 2mM DTT, 1mM EDTA 0.1M NaCl
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPEINGSAN YEMFIFHNGG VQILCKYPDI VQQFKMQLLK GGQILCDLTK TKGSGNTVSI KSLKFCHSQL SNNSVSFFLY NLDHSHANYY FCNLSIFDPP PFKVTLTGGY LHIYESQLCC QLKLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH
• Sequence Length: 199

• Applicable Applications for ICOS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ICOS recombinant protein

ICOS, also known as inducible T-cell costimulator, is a member of the CD28 family of immune-assisted stimulatory receptors. The interaction of B7-H2 / ICOS plays a crucial role in Th cell differentiation, T-B cell interaction and humoral immune response essential for the formation of reproductive centers and the production of cytokine IL-4. In addition, this protein is more potent in inducing IL-10 production, a cytokine important for the inhibitory function of T regulatory cells. The B7-1 / B7-2-CD28 / CTLA-4 and ICOS-B7RP-1 pathways provide an important second signal that can regulate the activation, inhibition and fine regulation of T-lymphocyte responses. It stimulates production of Th1 and Th2 cytokines, but may play a role in the generation of Th2 cells. Recombinant human ICOS, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ICOS recombinant protein

Immunology

References

Gonzalo, J.A., et al. (2001) J. Immunol. 166:1-5. Tezukak., et al. (2000) Biochem Biophys Res Commun. 276:335-345

NCBI and Uniprot Product Information

• NCBI GI #: 15029518
• NCBI GeneID: 29851
• NCBI Accession #: NP_036224
• NCBI GenBank Nucleotide #: NM_012092.3
• UniProt Accession #: Q9Y6W8; Q8N6W8
• Molecular Weight: 40.8kDa (362aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T cell costimulator
• NCBI Official Symbol: ICOS
• NCBI Official Synonym Symbols: AILIM; CD278; CVID1
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• Protein Family: ICOS ligand
• UniProt Gene Name: ICOS
• UniProt Synonym Gene Names: AILIM

NCBI Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes ().