ICOS/CD278 Active Protein | CD278 active protein

Human CellExp ICOS/CD278, Fc Tag, Human recombinant

Cat. Num. AAA8400002

• Gene Names: ICOS; AILIM; CD278; CVID1
• Purity: >95% as determined by SDS-PAGE.
• Synonyms: ICOS/CD278; Human CellExp ICOS/CD278; Fc Tag; Human recombinant; ICOS; CD278; AILIM; Inducible T-cell costimulator; CD278 active protein

• Host: HEK293 cells
• Purity/Purification: >95% as determined by SDS-PAGE.
• Form/Format: Lyophilized. Sterile Filtered colorless solution

• Gene Source: Human
• Solubility: Lyophilized from 0.22 um filtered solution in 50 mM Tris; 100 mM Glycine; 25 mM Arginine; 150 mM NaCl; pH7.5. Normally trehalose is added as protectant before lyophilization
• Biological Activity: Immobilized Human B7-H2, His Tag at 1 ug/mL (100 uL/well) can bind Human ICOS, Fc Tag with a linear range of 0.4-6 ng/mL
• Reconstitution Instruction: Reconstitute in sterile deionized water, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. It is recommended to store at store at -20 degree C.
• Tag Note: This protein carries a human IgG1 Fc tag at the C-terminus.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Testing Data

Related Product Information for CD278 active protein

Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278/ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

Product Categories/Family for CD278 active protein

Proteins; Enzymes; Cytokines; Growth Factors & Hormones; CDs; Proteins; Enzymes; Human cell expressed recombinant proteins

NCBI and Uniprot Product Information

• NCBI GI #: 15029518
• NCBI GeneID: 29851
• NCBI Accession #: NP_036224
• NCBI GenBank Nucleotide #: NM_012092.3
• UniProt Accession #: Q9Y6W8; Q8N6W8
• Molecular Weight: 40.8kDa (362aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T cell costimulator
• NCBI Official Symbol: ICOS
• NCBI Official Synonym Symbols: AILIM; CD278; CVID1
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• UniProt Gene Name: ICOS
• UniProt Synonym Gene Names: AILIM
• UniProt Entry Name: ICOS_HUMAN

NCBI Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane

Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation; immune response

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 1