ICOS elisa kit

Human ICOS PicoKine ELISA Kit

Cat. Num. AAA1751103

• Gene Names: ICOS; AILIM; CD278; CVID1
• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: ICOS; Human ICOS PicoKine ELISA Kit; Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD278; AILIM; ICOS elisa kit

• Reactivity: Human; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Human ICOS
• Sequence Length: 168

• Applicable Applications for ICOS elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <2pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ICOS elisa kit

Principle of the Assay: Human ICOS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICOS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E21-F141) and test samples are added to the wells, a biotinylated detection biotinylated polyclonal antibody from goat specific for ICOS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ICOS amount of sample captured in plate.

Background: Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 15029518
• NCBI GeneID: 29851
• NCBI Accession #: NP_036224.1
• NCBI GenBank Nucleotide #: NM_012092.3
• UniProt Accession #: Q9Y6W8; Q8N6W8
• Molecular Weight: 19,127 Da
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T cell costimulator
• NCBI Official Symbol: ICOS
• NCBI Official Synonym Symbols: AILIM; CD278; CVID1
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• Protein Family: ICOS ligand
• UniProt Gene Name: ICOS
• UniProt Synonym Gene Names: AILIM

NCBI Description

The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes ().