Human Apolipoprotein O (APOO) ELISA Kit | APOO elisa kit

Human Apolipoprotein O (APOO) ELISA Kit

Cat. Num. AAA453705

• Gene Names: APOO; MIC26; Mic23; My025; FAM121B
• Reactivity: Human
• Synonyms: Apolipoprotein O (APOO); Human Apolipoprotein O (APOO) ELISA Kit; Apo-O; FAM121B; My025; Family With Sequence Similarity 121B; APOO elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of APOO. No significant cross-reactivity or interference between APOO and analogues was observed.
• Sequence Length: 198

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.073ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APOO were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APOO were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOO elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of APOO in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APOO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APOO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APOO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APOO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13129148
• NCBI GeneID: 79135
• NCBI Accession #: NP_077027.1
• NCBI GenBank Nucleotide #: NM_024122.4
• UniProt Accession #: Q9BUR5; Q9H3J9; B2R4K9
• Molecular Weight: 20,199 Da
• NCBI Official Full Name: MICOS complex subunit MIC26
• NCBI Official Synonym Full Names: apolipoprotein O
• NCBI Official Symbol: APOO
• NCBI Official Synonym Symbols: MIC26; Mic23; My025; FAM121B
• NCBI Protein Information: MICOS complex subunit MIC26
• UniProt Protein Name: MICOS complex subunit MIC26
• Protein Family: MICOS complex
• UniProt Gene Name: APOO

NCBI Description

This gene is a member of the apolipoprotein family. Members of this protein family are involved in the transport and metabolism of lipids. The encoded protein associates with HDL, LDL and VLDL lipoproteins and is characterized by chondroitin-sulfate glycosylation. This protein may be involved in preventing lipid accumulation in the myocardium in obese and diabetic patients. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 4, 5, 12 and 16.[provided by RefSeq, Sep 2009]

Uniprot Description

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).