Rabbit HDAC4 Polyclonal Antibody | anti-HDAC4 antibody

HDAC4 Polyclonal Antibody

Cat. Num. AAA125481

• Gene Names: HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: HDAC4; Polyclonal Antibody; HDAC4 Polyclonal Antibody; HA6116; HD4; HDAC-A; HDACA; KIAA0288; anti-HDAC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 1084

• Applicable Applications for anti-HDAC4 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IF: 1:50 - 1:200
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Immunogen: A synthetic peptide of human HDAC4
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HDAC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells using HDAC4 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-HDAC4 antibody

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

Product Categories/Family for anti-HDAC4 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 259016348
• NCBI GeneID: 9759
• UniProt Accession #: P56524; Q86YH7; Q9UND6; E9PGB9; F5GX36
• Molecular Weight: Calculated MW: 106kDa/119kDa; Observed MW: 117kDa
• NCBI Official Full Name: Histone deacetylase 4
• NCBI Official Synonym Full Names: histone deacetylase 4
• NCBI Official Symbol: HDAC4
• NCBI Official Synonym Symbols: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• NCBI Protein Information: histone deacetylase 4; histone deacetylase A
• UniProt Protein Name: Histone deacetylase 4
• UniProt Gene Name: HDAC4
• UniProt Synonym Gene Names: KIAA0288; HD4
• UniProt Entry Name: HDAC4_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Protein type: Hydrolase; EC 3.5.1.98; Deacetylase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; transcriptional repressor complex; histone deacetylase complex; cytoplasm; actomyosin; neuromuscular junction; nucleus; Z disc; cytosol; A band

Molecular Function: potassium ion binding; transcription activator binding; zinc ion binding; histone deacetylase binding; protein deacetylase activity; protein kinase binding; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent histone deacetylase activity (H3-K14 specific); sequence-specific DNA binding; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity

Biological Process: cardiac muscle hypertrophy; response to drug; nervous system development; regulation of skeletal muscle fiber development; transcription, DNA-dependent; B cell activation; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; histone deacetylation; response to denervation involved in regulation of muscle adaptation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; osteoblast development; positive regulation of protein sumoylation; chromatin remodeling; negative regulation of cell proliferation; B cell differentiation; regulation of protein binding; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of osteoblast differentiation; negative regulation of transcription, DNA-dependent; negative regulation of glycolysis; inflammatory response; skeletal development