Class II HDAC Antibody Kit

Class II HDAC Antibody Sampler Kit

Cat. Num. AAA2550043

• Applications: Western Blot
• Synonyms: Class II HDAC; Class II HDAC Antibody Sampler Kit; Class II HDAC antibody kit

• Applicable Applications for Class II HDAC antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of Hela cells using HDAC4 Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of K562, 3T3 cells with HDAC5 Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells using HDAC6 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of PC-3 cells using HDAC7 Polyclonal Antibody at dilution of 1:1000.)

Related Product Information for Class II HDAC antibody kit

Background: Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes. In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity. Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases. Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents.