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SDS-PAGE (Figure annotation denotes ug of protein loaded and % gel used.)

HSP40/DNAJ recombinant protein

HSP40/DNAJ protein (His tag)

Gene Names
AT1G44160; T7O23.16; T7O23_16
Applications
SDS-Page
Purity
> 95% pure
Synonyms
HSP40/DNAJ; HSP40/DNAJ protein (His tag); radial spoke 16 homolog B (Chlamydomonas) protein; Heat shock protein 40 protein; DNAJ1 protein; HDJ-1 protein; HSP/DNAJ 40 protein; Heat shock 40 kDa protein 1 protein; Hsp40 protein; Sis1 protein; HSPF1 protein; DnaJ homolog subfamily B member 1 protein; HDJ1 protein; RSPH16B protein; DnaJ protein homolog 1 protein; HSP40 (DNAJ) protein; DNAJB1 protein; HSP/DNAJ-40; HSP/DNAJ 40; HSP/DNAJ-40 protein; HSP40 protein; HSP40/DNAJ recombinant protein
Ordering
For Research Use Only!
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in 20mM Tris, pH 8.0, containing 2mM DTT, 0.2mM PMSF, and 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
Sequence Length
357
Applicable Applications for HSP40/DNAJ recombinant protein
SDS-PAGE
Protein Type
Recombinant
Biological Significance
Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK. Recombinant human HSP40, his-tagged, is overexpressed in E Coli and purified by using the conventional column chromatography techniques.
Endotoxin Levels
< 1.0 EU per ug of protein (determined by LAL method)
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.

SDS-PAGE

(Figure annotation denotes ug of protein loaded and % gel used.)

SDS-PAGE (Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for HSP40/DNAJ recombinant protein
Purified recombinant Human HSP40/DNAJ protein
Product Categories/Family for HSP40/DNAJ recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40.2 kDa (360aa), confirmed by MALDI-TOF.
NCBI Official Full Name
HSP40/DnaJ peptide-binding protein
NCBI Official Symbol
AT1G44160
NCBI Official Synonym Symbols
T7O23.16; T7O23_16
NCBI Protein Information
HSP40/DnaJ peptide-binding protein

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Product Notes

The HSP40/DNAJ (Catalog #AAA536675) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSP40/DNAJ can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HSP40/DNAJ for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI. It is sometimes possible for the material contained within the vial of "HSP40/DNAJ, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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