Mouse anti-Human GARP Monoclonal Antibody | anti-GARP antibody

anti-human GARP (LRRC32)-R-PE

Cat. Num. AAA666736

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: GARP; Monoclonal Antibody; anti-human GARP (LRRC32)-R-PE; Monoclonal anti-human GARP (LRRC32)/R-PE; anti-GARP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2ak
• Clone Number: ANC8C9
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
• Concentration: Volume: 0.2 ml (varies by lot)
• Sequence Length: 662

• Applicable Applications for anti-GARP antibody: Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
• Performance: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD4/APC conjugate, and anti-human GARP/PE at dilution factor of 1:50 (10 ug/ml). Cells were washed twice, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 3.9% sub population of gated cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG2a/PE isotype control. This binding was blocked when cells were pre incubated with 50 ug/ml unlabeled anti-human GARP mAb.
• Conjugation: PE
• Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.5mg/ml with an A565/A280 ratiooof 2.63.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Recombinant human GARP
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-GARP antibody

Information: Human GARP (Glycoprotein A Repetitions Predominant/ Leucine Rich Repeat Containing 32) is a roughly 80 kd transmembrane cell surface molecule expressed on a variety of tissue types, including a subset of activated T regulatory cells. GARP covalently binds latent TGF-beta 1 serving as a regulatory reservoir(1). Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.

References

1) R Wang, T A Springer, et al. (2012) Mol Biol Cell 23(6): 1129-1139.

NCBI and Uniprot Product Information

• NCBI GI #: 439296
• UniProt Accession #: Q14392; Q86V06
• Molecular Weight: 71,979 Da
• NCBI Official Full Name: garp
• UniProt Protein Name: Leucine-rich repeat-containing protein 32
• Protein Family: Glutamic acid-rich protein
• UniProt Gene Name: LRRC32
• UniProt Synonym Gene Names: D11S833E; GARP; GARP
• UniProt Entry Name: LRC32_HUMAN

Uniprot Description

LRRC32:

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.5-q14

Cellular Component: integral to plasma membrane

Biological Process: negative regulation of activated T cell proliferation; negative regulation of cytokine secretion