HSP40 recombinant protein

HSP40 (DNAJ), 1-340 aa, Human, His tag, E Coli

Cat. Num. AAA203039

• Gene Names: DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HSP40; HSP40 (DNAJ); 1-340 aa; Human; His tag; E Coli; Heat shock 40 kDa protein 1; DNAJB1; DNAJ1; HDJ1; HSPF1; Heat shock protein 40; DnaJ protein homolog 1; HDJ-1; DnaJ homolog subfamily B member 1; radial spoke 16 homolog B (Chlamydomonas); Hsp40; Sis1; RSPH16B; ; HSP40 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris (pH 8.0), 2mM DTT, 0.2mMPMSF, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
• Sequence Length: 340

• Applicable Applications for HSP40 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for HSP40 recombinant protein

Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK. Recombinant human HSP40, his-tagged, is overexpressed in E Coli and purified by using the conventional column chromatography techniques.

Product Categories/Family for HSP40 recombinant protein

Heat Shock Proteins

References

Hata M, Okumura K, Seto M, Ohtsuka K. (1996). Genomics 38 (3): 446-9; Manishkumar and Debashis Mitra. (2005). Journal of Biological Chemistry 280 (48): 40041-40050.;

NCBI and Uniprot Product Information

• NCBI GI #: 5453690
• NCBI GeneID: 3337
• NCBI Accession #: NP_006136
• NCBI GenBank Nucleotide #: NM_006145.2
• UniProt Accession #: P25685; B4DX52
• Molecular Weight: 40.2kDa (360aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: dnaJ homolog subfamily B member 1 isoform 1
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B1
• NCBI Official Symbol: DNAJB1
• NCBI Official Synonym Symbols: Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• NCBI Protein Information: dnaJ homolog subfamily B member 1
• UniProt Protein Name: DnaJ homolog subfamily B member 1
• UniProt Gene Name: DNAJB1
• UniProt Synonym Gene Names: DNAJ1; HDJ1; HSPF1; HSP40; Heat shock protein 40; hDj-1

NCBI Description

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).