Western Blot (WB)
(The Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HSP40 Transfected 293T cell lysate)
Mouse anti-Human Hsp40 Monoclonal Antibody | anti-Hsp40 antibody
Hsp40 antibody
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-G affinity chromatography
Synonyms
Hsp40; Monoclonal Antibody; Hsp40 antibody; Heat shock 40 kDa protein 1; DNAJB1; DNAJ1; HDJ1; HSPF1; HSP40 (DNAJ); Heat shock protein 40; HSP40; DnaJ protein homolog 1; HDJ-1; DnaJ homolog subfamily B member 1; radial spoke 16 homolog B (Chlamydomonas); Sis1; RSPH16B; ; anti-Hsp40 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
k1C7
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
351
Applicable Applications for anti-Hsp40 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1,000 ~ 2,000.
Recommended starting dilution is 1:1,000.
Recommended starting dilution is 1:1,000.
Antigen Species
Human
Immunogen
Recombinant human Hsp40 (1-340aa) purified from E. coli
Clone Note
Anti-human Hsp40 mAb, clone k1C7, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp40 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HSP40 Transfected 293T cell lysate)
Western Blot (WB)
(The Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP40 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HSP40 Transfected 293T cell lysate)
Related Product Information for anti-Hsp40 antibody
Human Hsp40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. Hsp40 protein contains the J-domain which controls interaction with the ATPase domain of Dnak.
Product Categories/Family for anti-Hsp40 antibody
References
Hata M et al., (1996) Genomics 38 (3):446-449; Manishkumar et al., (2005) J Biol Chem 280 (48):40041-40050; Christine M et al., (2007) Genetics 175 (4):1649-1664
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,927 Da
NCBI Official Full Name
Hsp40
UniProt Protein Name
Hsp40
UniProt Gene Name
Hsp40
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Product Notes
The Hsp40 hsp40 (Catalog #AAA200058) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp40 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp40 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the Hsp40 hsp40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp40, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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