Typical Testing Data/Standard Curve (for reference only)
Rabbit Hemopexin ELISA Kit | HPX elisa kit
Rabbit Hemopexin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: HPX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HPX antibody and an HPX-HRP conjugate. The assay sample and buffer are incubated together with HPX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPX concentration since HPX from samples and HPX-HRP conjugate compete for the anti-HPX antibody binding site. Since the number of sites is limited, as more sites are occupied by HPX from the sample, fewer sites are left to bind HPX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPX concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]
Uniprot Description
HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11p15.5-p15.4
Cellular Component: extracellular space; extracellular region
Molecular Function: protein binding; metal ion binding; heme transporter activity
Biological Process: receptor-mediated endocytosis; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of tyrosine phosphorylation of Stat1 protein; heme transport; positive regulation of immunoglobulin production; viral reproduction; hemoglobin metabolic process; cellular iron ion homeostasis; heme metabolic process
Research Articles on HPX
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Product Notes
The Rabbit HPX hpx (Catalog #AAA720549) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720549 ELISA Kit recognizes Rabbit HPX. It is sometimes possible for the material contained within the vial of "Hemopexin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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