Rabbit Hemopexin ELISA Kit | HPX elisa kit

Rabbit Hemopexin ELISA Kit

Cat. Num. AAA720549

• Gene Names: HPX; HX
• Reactivity: Rabbit
• Synonyms: Hemopexin; Rabbit Hemopexin ELISA Kit; HPX elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HPX elisa kit

Intended Uses: This HPX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit HPX. This ELISA kit for research use only!

Principle of the Assay: HPX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HPX antibody and an HPX-HRP conjugate. The assay sample and buffer are incubated together with HPX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPX concentration since HPX from samples and HPX-HRP conjugate compete for the anti-HPX antibody binding site. Since the number of sites is limited, as more sites are occupied by HPX from the sample, fewer sites are left to bind HPX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPX concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HPX elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 184497
• NCBI GeneID: 3263
• Molecular Weight: 51,676 Da
• NCBI Official Full Name: hemopexin
• NCBI Official Synonym Full Names: hemopexin
• NCBI Official Symbol: HPX
• NCBI Official Synonym Symbols: HX
• NCBI Protein Information: hemopexin; beta-1B-glycoprotein
• UniProt Protein Name: Hemopexin
• Protein Family: Hemopexin
• UniProt Gene Name: HPX
• UniProt Entry Name: HEMO_HUMAN

NCBI Description

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]

Uniprot Description

HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; metal ion binding; heme transporter activity

Biological Process: receptor-mediated endocytosis; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of tyrosine phosphorylation of Stat1 protein; heme transport; positive regulation of immunoglobulin production; viral reproduction; hemoglobin metabolic process; cellular iron ion homeostasis; heme metabolic process