Mouse Prohibitin (PHB) ELISA Kit | PHB elisa kit

Mouse Prohibitin (PHB) ELISA Kit

Cat. Num. AAA2022334

• Gene Names: PHB; PHB1; HEL-215; HEL-S-54e
• Reactivity: Mouse
• Synonyms: Prohibitin (PHB); Mouse Prohibitin (PHB) ELISA Kit; PHB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prohibitin (PHB).; No significant cross-reactivity or interference between Prohibitin (PHB) and analogues was observed.
• Sequence Length: 155

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prohibitin (PHB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prohibitin (PHB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PHB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prohibitin (PHB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prohibitin (PHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prohibitin (PHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prohibitin (PHB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PHB elisa kit

Tumor immunity; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 246483
• NCBI GeneID: 5245
• UniProt Accession #: P35232; Q4VBQ0; B4DY47
• Molecular Weight: 17,018 Da
• NCBI Official Full Name: prohibitin
• NCBI Official Synonym Full Names: prohibitin
• NCBI Official Symbol: PHB
• NCBI Official Synonym Symbols: PHB1; HEL-215; HEL-S-54e
• NCBI Protein Information: prohibitin
• UniProt Protein Name: Prohibitin
• Protein Family: Prohibitin
• UniProt Gene Name: PHB
• UniProt Entry Name: PHB_HUMAN

NCBI Description

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.

Protein type: Mitochondrial; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Oncoprotein; Chaperone; Transcription, coactivator/corepressor; Cell cycle regulation; Apoptosis

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; mitochondrion; membrane; integral to plasma membrane; mitochondrial inner membrane; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; histone deacetylase binding

Biological Process: transcription from RNA polymerase II promoter; regulation of apoptosis; osteoblast differentiation; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; signal transduction

Disease: Breast Cancer