Human High mobility group protein HMG-I/HMG-Y ELISA Kit | HMGA1 elisa kit

Human High mobility group protein HMG-I/HMG-Y ELISA Kit

Cat. Num. AAA765994

• Gene Names: HMGA1; HMG-R; HMGIY; HMGA1A
• Reactivity: Human
• Synonyms: High mobility group protein HMG-I/HMG-Y; Human High mobility group protein HMG-I/HMG-Y ELISA Kit; HMGA1/High mobility group protein HMG-I/HMG-Y/HMG-I (Y))/High mobility group AT-hook protein 1/High mobility group protein A1/High mobility group protein R/HMGIY/HMG-R/HMG-Y; HMGA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HMGA1. No significant cross-reactivity or interference between HMGA1 and analogues was observed.
• Sequence Length: 107

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for HMGA1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 977380216
• NCBI GeneID: 3159
• NCBI Accession #: NP_001306011.1
• NCBI GenBank Nucleotide #: NM_001319082.1
• Molecular Weight: 19,694 Da
• NCBI Official Full Name: high mobility group protein HMG-I/HMG-Y isoform a
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: HMGA1
• NCBI Official Synonym Symbols: HMG-R; HMGIY; HMGA1A
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y
• UniProt Protein Name: High mobility group protein HMG-I/HMG-Y
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGA1
• UniProt Synonym Gene Names: HMGIY; HMG-I(Y); High mobility group protein A1
• UniProt Entry Name: HMGA1_HUMAN

NCBI Description

This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes. [provided by RefSeq, Jan 2016]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytosol; focal adhesion; nucleoplasm; nucleus; transcription factor complex

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; AT DNA binding; chromatin binding; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; peroxisome proliferator activated receptor binding; protein binding; retinoic acid receptor binding; retinoid X receptor binding; transcription factor activity; transcription factor binding

Biological Process: base-excision repair; DNA unwinding during replication; negative regulation of cell proliferation; negative regulation of chromatin silencing; negative regulation of transcription, DNA-dependent; nucleosome disassembly; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein complex assembly; regulation of transcription, DNA-dependent; response to virus; transcription from RNA polymerase II promoter; viral reproduction