Human high mobility group protein 17 (HMG-17) ELISA Kit | HMG-17 elisa kit

Human high mobility group protein 17 (HMG-17) ELISA Kit

Cat. Num. AAA269459

• Gene Names: HMGA1; HMG-R; HMGIY; HMGA1A
• Reactivity: Human
• Synonyms: high mobility group protein 17 (HMG-17); Human high mobility group protein 17 (HMG-17) ELISA Kit; HMG-17 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 107

• Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Hla-G Concentration
• Assay Type: Sandwich
• Detection Range: 200 U/ml-3.12 U/ml
• Sensitivity: 0.6U/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HMG-17 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HLA-G monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 22208971
• NCBI GeneID: 3159
• NCBI Accession #: NP_665908.1
• NCBI GenBank Nucleotide #: NM_145901.2
• Molecular Weight: 19,694 Da
• NCBI Official Full Name: high mobility group protein HMG-I/HMG-Y isoform a
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: HMGA1
• NCBI Official Synonym Symbols: HMG-R; HMGIY; HMGA1A
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y; HMG-I(Y); high mobility group protein R; high mobility group protein A1; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; high-mobility group (nonhistone chromosomal) protein isoforms I and Y
• UniProt Protein Name: High mobility group protein HMG-I/HMG-Y
• UniProt Gene Name: HMGA1
• UniProt Synonym Gene Names: HMGIY; HMG-I(Y); High mobility group protein A1
• UniProt Entry Name: HMGA1_HUMAN

NCBI Description

This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; transcription factor complex; focal adhesion; nucleus; cytosol

Molecular Function: retinoid X receptor binding; peroxisome proliferator activated receptor binding; protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; AT DNA binding; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding

Biological Process: DNA unwinding during replication; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; response to virus; DNA catabolic process, endonucleolytic; negative regulation of cell proliferation; nucleosome disassembly; regulation of transcription, DNA-dependent; negative regulation of chromatin silencing; base-excision repair; protein complex assembly; negative regulation of transcription, DNA-dependent