SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
RIOK1 recombinant protein
RIOK1 Protein
Gene Names
RIOK1; AD034; RRP10; bA288G3.1
Applications
Western Blot
Synonyms
RIOK1; RIOK1 Protein; AD034; RRP10; bA288G3.1; FLJ30006; MGC12903; RIOK1 recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for RIOK1 recombinant protein
Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for RIOK1 recombinant protein
Full-length recombinant human RIOK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. This gene accession number is BC006104.
RIOK1 or RIO kinase 1 is a novel cytoplasmic component of pre-40S pre-ribosomal particle(s) in human cells which is required in the final stages of cytoplasmic pre-40S maturation (1). RioK1 is a new interactor of protein arginine methyltransferase 5 (PRMT5) which competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity (2). RIOK1 may reveal new insights into defects underlying glioblastoma and related cancers and may reveal new therapeutic opportunities for these cancers.
RIOK1 or RIO kinase 1 is a novel cytoplasmic component of pre-40S pre-ribosomal particle(s) in human cells which is required in the final stages of cytoplasmic pre-40S maturation (1). RioK1 is a new interactor of protein arginine methyltransferase 5 (PRMT5) which competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity (2). RIOK1 may reveal new insights into defects underlying glioblastoma and related cancers and may reveal new therapeutic opportunities for these cancers.
Product Categories/Family for RIOK1 recombinant protein
References
1. Widmann B. et.al: The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits. Mol Biol Cell. 2012 Jan;23(1):22-35. doi: 10.1091/mbc.E11-07-0639. Epub 2011 Nov 9.
2. Guderian G1. et.al: RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. J Biol Chem. 2011 Jan 21;286(3):1976-86. doi: 10.1074/jbc.M110.148486. Epub 2010 Nov 16.
2. Guderian G1. et.al: RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. J Biol Chem. 2011 Jan 21;286(3):1976-86. doi: 10.1074/jbc.M110.148486. Epub 2010 Nov 16.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
65,583 Da
NCBI Official Full Name
Homo sapiens RIO kinase 1 (yeast), mRNA
NCBI Official Synonym Full Names
RIO kinase 1
NCBI Official Symbol
RIOK1
NCBI Official Synonym Symbols
AD034; RRP10; bA288G3.1
NCBI Protein Information
serine/threonine-protein kinase RIO1
NCBI Description
This gene includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. [provided by RefSeq, Jul 2008]
Research Articles on RIOK1
Similar Products
Product Notes
The RIOK1 (Catalog #AAA517522) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RIOK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the RIOK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIOK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
