Typical Testing Data/Standard Curve (for reference only)
Human HLA class I histocompatibility antigen, alpha chain E ELISA Kit | HLA-E elisa kit
Human HLA class I histocompatibility antigen, alpha chain E ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA class I histocompatibility antigen, alpha chain E. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHLA class I histocompatibility antigen, alpha chain E and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HLA class I histocompatibility antigen, alpha chain E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HLA class I histocompatibility antigen, alpha chain E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Uniprot Description
HLA-E: Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. Belongs to the MHC class I family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cell surface; early endosome membrane; Golgi membrane; MHC class I protein complex; phagocytic vesicle membrane; plasma membrane
Molecular Function: beta-2-microglobulin binding; MHC class I protein binding; natural killer cell lectin-like receptor binding; peptide antigen binding; receptor binding
Biological Process: adaptive immune response; antibacterial humoral response; antigen processing and presentation of endogenous peptide antigen via MHC class Ib; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; defense response to Gram-positive bacterium; innate immune response; positive regulation of immunoglobulin secretion; positive regulation of interleukin-13 production; positive regulation of interleukin-4 production; positive regulation of natural killer cell mediated immunity; positive regulation of T cell mediated cytotoxicity; positive regulation of TRAIL production; positive regulation of tumor necrosis factor production; protection from natural killer cell mediated cytotoxicity; regulation of immune response; regulation of natural killer cell mediated immunity
Research Articles on HLA-E
Similar Products
Product Notes
The Human HLA-E hla-e (Catalog #AAA2885177) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885177 ELISA Kit recognizes Human HLA-E. It is sometimes possible for the material contained within the vial of "HLA class I histocompatibility antigen, alpha chain E, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
