Typical Testing Data/Standard Curve (for reference only)
Human MHCE/HLA-E (Major Histocompatibility Complex Class I E) CLIA Kit | MHCE/HLA-E clia kit
Human MHCE/HLA-E (Major Histocompatibility Complex Class I E) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to MHCE/HLA-E. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MHCE/HLA-E and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MHCE/HLA-E, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of MHCE/HLA-E. You can calculate the concentration of MHCE/HLA-E in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Uniprot Description
HLA-E: Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. Belongs to the MHC class I family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; early endosome membrane; plasma membrane; MHC class I protein complex
Molecular Function: peptide antigen binding; beta-2-microglobulin binding; MHC class I protein binding; natural killer cell lectin-like receptor binding; receptor binding
Biological Process: adaptive immune response; regulation of immune response; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; regulation of natural killer cell mediated immunity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of immunoglobulin secretion; positive regulation of TRAIL production; positive regulation of tumor necrosis factor production; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of endogenous peptide antigen via MHC class Ib; positive regulation of interleukin-4 production; antigen processing and presentation of peptide antigen via MHC class I; defense response to Gram-positive bacterium; positive regulation of natural killer cell mediated immunity; antibacterial humoral response; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; positive regulation of interleukin-13 production
Research Articles on MHCE/HLA-E
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Product Notes
The Human MHCE/HLA-E hla-e (Catalog #AAA2531395) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531395 CLIA Kit recognizes Human MHCE/HLA-E. It is sometimes possible for the material contained within the vial of "MHCE/HLA-E (Major Histocompatibility Complex Class I E), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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