Rabbit anti-Human, Mouse USF1 Polyclonal Antibody | anti-USF1 antibody

USF1 Polyclonal Antibody

Cat. Num. AAA3008178

• Gene Names: USF1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: USF1; Polyclonal Antibody; USF1 Polyclonal Antibody; bHLHb11; Class B basic helix-loop-helix protein 11; FCHL 1; FCHL; FCHL1; HGNC: 12593; HYPLIP 1; HYPLIP1; Major late transcription factor 1; Major late transcription factor; MLTF; MLTF I; MLTFI; Transcription factor USF-1; UEF; Upstream stimulatory factor 1; Upstream transcription factor 1; isoform CRA_a; USF 1; USF; anti-USF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: USF1 polyclonal antibody detects endogenous levels of USF1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 310

• Applicable Applications for anti-USF1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human USF1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of USF1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney cancer using USF1 antibody.)

Testing Data

Related Product Information for anti-USF1 antibody

The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of (USF-1) and (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.

NCBI and Uniprot Product Information

• NCBI GI #: 137170
• NCBI GeneID: 7391
• UniProt Accession #: P22415
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: Upstream stimulatory factor 1
• NCBI Official Synonym Full Names: upstream transcription factor 1
• NCBI Official Symbol: USF1
• NCBI Official Synonym Symbols: UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
• NCBI Protein Information: upstream stimulatory factor 1
• UniProt Protein Name: Upstream stimulatory factor 1
• Protein Family: Upstream stimulatory factor
• UniProt Gene Name: USF1
• UniProt Synonym Gene Names: BHLHB11; USF; bHLHb11
• UniProt Entry Name: USF1_HUMAN

NCBI Description

This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]

Uniprot Description

USF1: Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: nucleoplasm; transcription factor complex; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; lipid homeostasis; glucose metabolic process; late viral mRNA transcription; glucose homeostasis; regulation of transcription from RNA polymerase II promoter; negative regulation of fibrinolysis; cellular response to insulin stimulus; regulation of transcription from RNA polymerase II promoter by glucose; response to hypoxia; regulation of transcription by carbon catabolites; positive regulation of transcription from RNA polymerase II promoter by glucose; positive regulation of transcription from RNA polymerase II promoter; response to UV

Disease: Hyperlipidemia, Combined, 1