Rat hepatocyte growth factor (hepapoietin A; scatter factor) ELISA Kit | HGF elisa kit

Rat hepatocyte growth factor, HGF ELISA Kit

Cat. Num. AAA701555

• Gene Names: Hgf; HPTA
• Reactivity: Rat
• Synonyms: hepatocyte growth factor (hepapoietin A; scatter factor); Rat hepatocyte growth factor; HGF ELISA Kit; HGF ELISA kit; DFNB39; F-TCF; HGFB; HPTA; SF; OTTHUMP00000206710; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor; lung fibroblast-derived mitogen; HGF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat HGF. No significant cross-reactivity or interference between Rat HGF and analogues was observed.
• Sequence Length: 728

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGF elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8393526
• NCBI GeneID: 24446
• NCBI Accession #: NP_058713.1
• NCBI GenBank Nucleotide #: NM_017017.2
• UniProt Accession #: P17945
• Molecular Weight: 82,906 Da
• NCBI Official Full Name: hepatocyte growth factor preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor
• NCBI Official Symbol: Hgf
• NCBI Official Synonym Symbols: HPTA
• NCBI Protein Information: hepatocyte growth factor; SF; scatter factor; hepatopoeitin-A; hepatopoietin-A
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: Hgf
• UniProt Synonym Gene Names: SF
• UniProt Entry Name: HGF_RAT

NCBI Description

plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle [RGD, Feb 2006]

Uniprot Description

Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization