Human hepatocyte growth factor (hepapoietin A; scatter factor) ELISA Kit | HGF elisa kit

Human hepatocyte growth factor, HGF ELISA Kit

Cat. Num. AAA705107

• Gene Names: HGF; SF; HGFB; HPTA; F-TCF; DFNB39
• Reactivity: Human
• Synonyms: hepatocyte growth factor (hepapoietin A; scatter factor); Human hepatocyte growth factor; HGF ELISA Kit; HGF ELISA kit; DFNB39; F-TCF; HGFB; HPTA; SF; OTTHUMP00000206710; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor; lung fibroblast-derived mitogen; HGF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human HGF. No significant cross-reactivity or interference between Human HGF and analogues was observed.
• Sequence Length: 728

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 6.25 pg/ml-400 pg/ml
• Sensitivity: 1.56 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGF elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 33859835
• NCBI GeneID: 3082
• NCBI Accession #: NP_000592.3
• NCBI GenBank Nucleotide #: NM_000601.4
• UniProt Accession #: P14210; Q02935; Q13494; Q14519; Q3KRB2; Q8TCE2; Q9BYL9; Q9BYM0; Q9UDU6; A1L3U6
• Molecular Weight: 83,134 Da
• NCBI Official Full Name: hepatocyte growth factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor (hepapoietin A; scatter factor)
• NCBI Official Symbol: HGF
• NCBI Official Synonym Symbols: SF; HGFB; HPTA; F-TCF; DFNB39
• NCBI Protein Information: hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: HGF
• UniProt Synonym Gene Names: HPTA; SF
• UniProt Entry Name: HGF_HUMAN

NCBI Description

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.2 PublicationsManual assertion based on experiment in:Ref.23