Rat HGF ELISA Kit | HGF elisa kit

Rat HGF PicoKine ELISA Kit

Cat. Num. AAA177216

• Gene Names: Hgf; HPTA
• Reactivity: Rat
• Synonyms: HGF; Rat HGF PicoKine ELISA Kit; Hepatocyte growth factor; hepatocyte growth factor (hepapoietin A; scatter factor); DFNB39; F TCF; Fibroblast derived tumor cytotoxic factor; Hepapoietin A; Hepatocyte growth factor (hepapoietin A scatter factor; Hepatocyte growth factor beta chain; Hepatopoietin-A; Hgf; HGF_HUMAN; HGFB; HPTA; Lung fibroblast derived mitogen; Scatter factor; SF; HGF elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Hgf
• Sequence Length: 728

• Samples: Cell culture supernates, serum and plasma(edta, citrate)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGF elisa kit

Principle of the Assay: The Picokine™ Rat Hgf Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Hgf with a 96-well strip plate that is pre-coated with antibody specific for Hgf. The detection antibody is a biotinylated antibody specific for Hgf. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Hgf with immunogen: Expression system for standard: NSO; Immunogen sequence: Q33-R495(alpha) & V496-L728(beta). The kit is analytically validated with ready to use reagents. To measure Rat Hgf, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat Hgf in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Hgf in the sample.

Background/Introduction: Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000.1 The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus.2 HGF may serve as a paracrine mediator to control placental development and growth.3 This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin.4 The gene encoding the human HGF is assigned to human chromosome 7.

NCBI and Uniprot Product Information

• NCBI GI #: 123117
• NCBI GeneID: 24446
• UniProt Accession #: P17945
• Molecular Weight: 82,906 Da
• NCBI Official Full Name: Hepatocyte growth factor
• NCBI Official Synonym Full Names: hepatocyte growth factor
• NCBI Official Symbol: Hgf
• NCBI Official Synonym Symbols: HPTA
• NCBI Protein Information: hepatocyte growth factor; SF; scatter factor; hepatopoeitin-A; hepatopoietin-A
• UniProt Protein Name: Hepatocyte growth factor
• Protein Family: Hepatocyte growth factor
• UniProt Gene Name: Hgf
• UniProt Synonym Gene Names: SF
• UniProt Entry Name: HGF_RAT

NCBI Description

plays a role in positive regulation of cell proliferation; may promote entry into the cell cycle [RGD, Feb 2006]

Uniprot Description

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization ().