TL1A Active Protein | TNFSF15 active protein

TL1A Protein, Human, Recombinant (rFc Tag)

Cat. Num. AAA8122089

• Gene Names: TNFSF15; TL1; TL1A; VEGI; VEGI192A
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TL1A; TL1A Protein; Human; Recombinant (rFc Tag); Human TL1A/TNFSF15 Protein (rFc Tag); TL1 Protein; VEGI Protein; VEGI192A Protein; tumor necrosis factor (ligand) superfamily; member 15; TNFSF15 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Leu72-Leu251

• Species: Human
• Activity: Measured by its ability to induce apoptosis of TF1 human erythroleukemic cells. The ED₅₀for this effect is typically 20-120ng/mL.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ser
• Tag: N-rabbit IgG-Fc
• Protein Construction: A DNA sequence encoding the human TNFSF15 (NP_005109.2)(Leu72-Leu251) was expressed with the Fc region of rabbit IgG at the N-terminus..
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to induce apoptosis of TF1 human erythroleukemic cells.The ED50 for this effect is typically 20-120 ng/mL.)

Related Product Information for TNFSF15 active protein

Background: TL1A, also known as TNFSF15, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is specifically expressed in endothelial cells. TL1A also can be detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. TL1A is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It mediates activation of NF-kappa-B. It also inhibits vascular endothelial growth and angiogenesis (in vitro). TL1A promotes activation of caspases and apoptosis. It is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor.

Product Categories/Family for TNFSF15 active protein

Death Receptor Signaling

References

Jin T, et al. (2007) X-ray crystal structure of TNF ligand family member TL1A at 2.1A. Biochem Biophys Res Commun. 364(1):1-6.Cassatella MA, et al. (2007) Soluble TNF-like cytokine (TL1A) production by immune complexes stimulated monocytes in rheumatoid arthritis. J Immunol. 178(11):7325-33Prehn JL, et al. (2007) The T cell costimulator TL1A is induced by FcgammaR signaling in human monocytes and dendritic cells. J Immunol. 178(7):4033-8.

NCBI and Uniprot Product Information

• NCBI GI #: 23510445
• NCBI GeneID: 9966
• NCBI Accession #: NP_005109.2
• NCBI GenBank Nucleotide #: NM_005118.3
• UniProt Accession #: O95150; Q3SX69; Q5VJK8; Q5VWH1; Q8NFE9
• Molecular Weight: 20,131 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 15 isoform VEGI-251
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 15
• NCBI Official Symbol: TNFSF15
• NCBI Official Synonym Symbols: TL1; TL1A; VEGI; VEGI192A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 15; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 15
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF15
• UniProt Synonym Gene Names: TL1; VEGI
• UniProt Entry Name: TNF15_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFSF15: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; cytokine metabolic process; activation of NF-kappaB-inducing kinase; immune response; signal transduction; positive regulation of cytokine secretion