Rabbit anti-Human TNFSF15 Polyclonal Antibody | anti-TNFSF15 antibody

TNFSF15, ID (TNFSF15, TL1, VEGI, Tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1, Vascular endothelial cell growth inhibitor, Tumor necrosis factor ligand superfamily member 15, membrane form, Tumor necrosis factor ligand

Cat. Num. AAA6357047

• Gene Names: TNFSF15; TL1; TL1A; VEGI; VEGI192A
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TNFSF15; Polyclonal Antibody; ID (TNFSF15; TL1; VEGI; Tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor; membrane form; Tumor necrosis factor ligand; secreted form; anti-TNFSF15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TNFSF15.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TNFSF15 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 148-175 in the central region of human TNFSF15.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using MBS644126 Lane 1: nontransfected Lane 2: transiently transfected with the TNFSF15 gene.)

Related Product Information for anti-TNFSF15 antibody

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. An additional isoform encoded by an alternatively spliced transcript variant has been reported but the sequence of this transcript has not been determined. [provided by RefSeq].

Product Categories/Family for anti-TNFSF15 antibody

Antibodies; Growth Factors; Cytokines

References

1. Amre, D.K., et al. Hum. Genet. 128(2):131-135(2010). 2. Heidemann, S.C., et al. J. Clin. Immunol. 30(4):531-538(2010). 3. McLaren, J.E., et al. J. Immunol. 184(10):5827-5834(2010). 4. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 5. Nakagome, S., et al. Ann. Hum. Genet. 74(2):126-136(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 23510445
• NCBI GeneID: 9966
• NCBI Accession #: NP_005109.2
• NCBI GenBank Nucleotide #: NM_005118.3
• UniProt Accession #: O95150; Q3SX69; Q5VJK8; Q5VWH1; Q8NFE9
• Molecular Weight: 20,131 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 15 isoform VEGI-251
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 15
• NCBI Official Symbol: TNFSF15
• NCBI Official Synonym Symbols: TL1; TL1A; VEGI; VEGI192A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 15; TNF superfamily ligand TL1A; TNF ligand-related molecule 1; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 15
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF15
• UniProt Synonym Gene Names: TL1; VEGI
• UniProt Entry Name: TNF15_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFSF15: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; cytokine metabolic process; activation of NF-kappaB-inducing kinase; immune response; signal transduction; positive regulation of cytokine secretion