Rabbit anti-Human KAT5 Polyclonal Antibody | anti-KAT5 antibody

KAT5 Antibody (C-Term)

Cat. Num. AAA9216278

• Gene Names: KAT5; TIP; ESA1; PLIP; TIP60; cPLA2; HTATIP; ZC2HC5; HTATIP1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: KAT5; Polyclonal Antibody; KAT5 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Histone acetyltransferase KAT5; 60 kDa Tat-interactive protein; Tip60; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA(2)-interacting protein; HTATIP; TIP60; anti-KAT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This KAT5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 367-398 amino acids from human KAT5.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 494

• Applicable Applications for anti-KAT5 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-KAT5 Antibody (C-Term) at 1:2000 dilutionLane 1: PC-3 whole cell lysateLane 2: SK-BR-3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-KAT5 antibody

Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2.

Product Categories/Family for anti-KAT5 antibody

Immunology; Microbiology

References

Kamine J.,et al.Virology 216:357-366(1996). Sheridan A.M.,et al.Mol. Cell. Biol. 21:4470-4481(2001). Legube G.,et al.Gene 310:161-168(2003). Ota T.,et al.Nat. Genet. 36:40-45(2004). Taylor T.D.,et al.Nature 440:497-500(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 332205931
• NCBI GeneID: 10524
• NCBI Accession #: NP_001193762.1
• NCBI GenBank Nucleotide #: NM_001206833.1
• UniProt Accession #: Q92993; O95624; Q13430; Q17RW5; Q561W3; Q6GSE8; Q9BWK7; B4E3C7; C9JL99
• Molecular Weight: 58582
• NCBI Official Full Name: histone acetyltransferase KAT5 isoform 4
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 5
• NCBI Official Symbol: KAT5
• NCBI Official Synonym Symbols: TIP; ESA1; PLIP; TIP60; cPLA2; HTATIP; ZC2HC5; HTATIP1
• NCBI Protein Information: histone acetyltransferase KAT5
• UniProt Protein Name: Histone acetyltransferase KAT5
• Protein Family: 3-ketoacyl-CoA thiolase
• UniProt Gene Name: KAT5
• UniProt Synonym Gene Names: HTATIP; TIP60; Tip60; HIV-1 Tat interactive protein
• UniProt Entry Name: KAT5_HUMAN

NCBI Description

The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Tip60: a histone acetyl transferase (HAT) of the MYST family. Originally isolated as an HIV-1 TAT-interactive protein. Plays an important role in regulating chromatin remodeling, transcription and other nuclear processes by acetylating nuclear proteins. Plays a role in DNA repair and apoptosis. Three splice variants have been described.

Protein type: EC 2.3.1.48; Nuclear receptor co-regulator; Acetyltransferase; Nucleolus

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; NuA4 histone acetyltransferase complex; perinuclear region of cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; histone acetyltransferase activity; androgen receptor binding; metal ion binding; transcription coactivator activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of interleukin-2 production; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; double-strand break repair; regulation of growth; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; response to ionizing radiation; histone acetylation; negative regulation of transcription, DNA-dependent