Semaphorin 4D Cell Lysate | SEMA4D cell lysate

Mouse Semaphorin 4D HEK293 Overexpression Lysate

Cat. Num. AAA8116758

• Gene Names: Sema4d; CD100; Semaj; Semcl2; coll-4; Semacl2
• Applications: Western Blot
• Synonyms: Semaphorin 4D; Mouse Semaphorin 4D HEK293 Overexpression Lysate; Mouse Semaphorin 4D/SEMA4D/CD100 HEK293 Cell Lysate (WB positive control); semaphorin 4D; Mouse CD100 Overexpression Lysate; Mouse coll-4 Overexpression Lysate; Mouse Semacl2 Overexpression Lysate; Mouse Semaj Overexpression Lysate; Mouse Semcl2 Overexpression Lysate; SEMA4D cell lysate

• Host: HEK293 Cells
• Form/Format: 1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).; Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Sequence Positions: 721aa
• Sequence: Met1-Arg733

Applicable Applications for SEMA4D cell lysate

Western Blot (WB)

Application Notes

WB: Use at an assay dependent dilution.

Species

Mouse

Recommend Usage

1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.

Sequence Construction

A DNA sequence encoding the mouse SEMA4D (NP_038688.2) extracellular domain (Met 1-Arg 733) was expressed, with a C-terminal polyhistidine tag.

Preparation Method

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.

Preparation and Storage

Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.