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Flow Cytometry (FC/FACS) (Flow cytometric analysis of mouse SEMA4D (CD100) expression on splenocytes.)

Rabbit anti-Mouse Semaphorin 4D Monoclonal Antibody | anti-SEMA4D antibody

Recombinant Anti-Semaphorin 4D Antibody (FITC), Rabbit Monoclonal

Gene Names
Sema4d; CD100; Semaj; Semcl2; coll-4; Semacl2
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
Semaphorin 4D; Monoclonal Antibody; Recombinant Anti-Semaphorin 4D Antibody (FITC); Rabbit Monoclonal; Semaphorin 4D/SEMA4D/CD100 Antibody (FITC); Rabbit MAb; semaphorin 4D; Anti-CD100 Antibody; Anti-coll-4 Antibody; Anti-Semacl2 Antibody; Anti-Semaj Antibody; Anti-Semcl2 Antibody; anti-SEMA4D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
010
Specificity
Mouse Semaphorin 4D/SEMA4D/CD100
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-SEMA4D antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse Semaphorin 4D/SEMA4D/CD100 protein
Conjugation
FITC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Semaphorin 4D/SEMA4D/CD100 (rM Semaphorin 4D/SEMA4D/CD100) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of mouse SEMA4D (CD100) expression on splenocytes.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of mouse SEMA4D (CD100) expression on splenocytes.)
Related Product Information for anti-SEMA4D antibody
Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95,640 Da
NCBI Official Full Name
semaphorin-4D
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
Sema4d
NCBI Official Synonym Symbols
CD100; Semaj; Semcl2; coll-4; Semacl2
NCBI Protein Information
semaphorin-4D
UniProt Protein Name
Semaphorin-4D
Protein Family
UniProt Gene Name
Sema4d
UniProt Synonym Gene Names
Semacl2; Semaj; Sema J

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13|13 A5

Cellular Component: extracellular space; integral to plasma membrane; membrane; plasma membrane

Molecular Function: chemorepellent activity; protein binding; receptor activity; receptor binding; semaphorin receptor binding; transmembrane receptor activity

Biological Process: negative chemotaxis; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; negative regulation of osteoblast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; positive regulation of axonogenesis; positive regulation of cell migration; positive regulation of collateral sprouting; positive regulation of GTPase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; regulation of cell projection organization and biogenesis; regulation of cell shape; regulation of dendrite morphogenesis

Research Articles on SEMA4D

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Product Notes

The SEMA4D sema4d (Catalog #AAA8111870) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Semaphorin 4D Antibody (FITC), Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 4D can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the SEMA4D sema4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Semaphorin 4D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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