Semaphorin 4D Active Protein | Sema4d active protein

Recombinant Mouse Semaphorin 4D Protein

Cat. Num. AAA2556767

• Gene Names: Sema4d; CD100; Semaj; Semcl2; coll-4; Semacl2
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Semaphorin 4D; Recombinant Mouse Semaphorin 4D Protein; SEMA4D; Semaphorin-4D; M-Sema G; Semaphorin-C-like 2; Semaphorin-J; Sema J; CD100; Semacl2; Semaj; Sema4d active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence Length: 861

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Arg 733
• Fusion Tag: C-Fc
• Activity: Measured by its ability to bind human SEMA4A in a functional ELISA.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

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Related Product Information for Sema4d active protein

Protein Construction: Recombinant Mouse Semaphorin 4D is produced by our Mammalian expression system and the target gene encoding Phe24-Met711 is expressed with a Fc tag at the C-terminus.

Background: SEMA4D is a member of the semaphorin family,contains one Ig-like C2-type domain, one PSI domain and one Sema domain. SEMA4D is strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues. However, SEMA4D is expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. SEMA4D is a cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. SEMA4D is involved in a number of fundamental biological processes such as promoting reorganization of the actin cytoskeleton, the migration of cerebellar granule cells and of endothelial cells and signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Not only these, it plays a role in axonal growth cone guidance in the developing central nervous system. Semaphorin-4D/SEMA4D may play a functional role in the immune system, as well as in the nervous system. It could induce B-cells to aggregate and improves their viability (in vitro). SEMA4D is involved in regulating dendrite and axon branching and morphogenesis and promoting interaction with PLXNB1 mediates activation of RHOA.

NCBI and Uniprot Product Information

• NCBI GI #: 49274623
• NCBI GeneID: 20354
• NCBI Accession #: NP_038688.2
• NCBI GenBank Nucleotide #: NM_013660.4
• UniProt Accession #: O09126; Q6GTM9
• Molecular Weight: Molecular Mass: 103.4kDa; Actual Protein Molecular Mass: 130 & 170kDa
• NCBI Official Full Name: semaphorin-4D
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• NCBI Official Symbol: Sema4d
• NCBI Official Synonym Symbols: CD100; Semaj; Semcl2; coll-4; Semacl2
• NCBI Protein Information: semaphorin-4D
• UniProt Protein Name: Semaphorin-4D
• Protein Family: Semaphorin
• UniProt Gene Name: Sema4d
• UniProt Synonym Gene Names: Semacl2; Semaj; Sema J

Uniprot Description

Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA ().