Rabbit anti-Human TXNRD1 Polyclonal Antibody | anti-TXNRD1 antibody

TXNRD1 (Thioredoxin Reductase 1, Cytoplasmic, Thioredoxin Reductase TR1, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, Gene Associated with Retinoic and IFN-induced Mortality 12 Protein, GRIM12, GRIM-12, KM-102-derived Red

Cat. Num. AAA647299

• Gene Names: TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TXNRD1; Polyclonal Antibody; TXNRD1 (Thioredoxin Reductase 1; Cytoplasmic; Thioredoxin Reductase TR1; TR; Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein; Gene Associated with Retinoic and IFN-induced Mortality 12 Protein; GRIM12; GRIM-12; KM-102-derived Red; Anti -TXNRD1 (Thioredoxin Reductase 1; anti-TXNRD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TXNRD1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MNGPEDLPKSYDYDLIIIGGGSGGLAAAKEAAQYGKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALQDSRNYGWKVEETVKHDWDRMIEAVQNHIGSLNWGYRVALREKKVVYENAYGQFIGPHRIKATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGC

• Applicable Applications for anti-TXNRD1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human TXNRD1, aa1-497 (NP_877393.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TXNRD1 rabbit polyclonal antibody. Western Blot analysis of TXNRD1 expression in human kidney.)

Western Blot (WB)

(TXNRD1 rabbit polyclonal antibody. Western Blot analysis of TXNRD1 expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of TXNRD1 expression in transfected 293T cell line by TXNRD1 polyclonal antibody. Lane 1: TXNRD1 transfected lysate (54.5kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-TXNRD1 antibody

Antibodies; Abs to Thioredoxin

NCBI and Uniprot Product Information

• NCBI GI #: 387598071
• NCBI GeneID: 7296
• NCBI Accession #: NP_001248375.1
• NCBI GenBank Nucleotide #: NM_001261446.1
• UniProt Accession #: Q16881; Q6FI31; Q6VB40; Q6VB41; Q6VB42; Q6VBP2; Q6VBP3; Q6VBP4; Q6VBP5; Q6VBP9; Q6VBQ0; Q6YNQ1
• Molecular Weight: 70,906 Da
• NCBI Official Full Name: thioredoxin reductase 1, cytoplasmic isoform 5
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: TXNRD1
• NCBI Official Synonym Symbols: TR; TR1; TXNR; TRXR1; GRIM-12
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic; oxidoreductase; thioredoxin reductase TR1; thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12
• UniProt Entry Name: TRXR1_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. Ref.3 Ref.12 Ref.14 Ref.17

Catalytic activity: Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.

Cofactor: Binds 1 FAD per subunit.

Subunit structure: Homodimer. Isoform 4 interacts with ESR1 and ESR2. Interacts with HERC5. Ref.12 Ref.14 Ref.16

Subcellular location: Cytoplasm

By similarity Ref.14. Isoform 4: Cytoplasm Ref.14. Nucleus Ref.14. Isoform 5: Cytoplasm Ref.14.

Tissue specificity: Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver. Ref.2 Ref.14 Ref.17

Induction: Isoform 5 is induced by a combination of interferon-beta and retinoic acid (at protein level). Isoform 1 is induced by estradiol or testosterone in HeLa cells. Ref.3 Ref.17

Domain: The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays. Ref.13

Post-translational modification: The N-terminus of isoform 5 is blocked.ISGylated

Probable. Ref.16

Miscellaneous: The thioredoxin reductase active site is a redox-active disulfide bond. The selenocysteine residue is also essential for catalytic activity.

Sequence similarities: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.Contains 1 glutaredoxin domain.

Sequence caution: The sequence AAB35418.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence AAC69621.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence AAF15900.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence AAV38446.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence AAZ67916.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence BAA13674.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence CAA04503.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence CAA62629.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.The sequence CAG38744.1 differs from that shown. Reason: Erroneous termination at position 648. Translated as Sec.