PLRP2 Cell Lysate | PNLIPRP2 cell lysate
Human PLRP2 HEK293 Overexpression Lysate
Gene Names
PNLIPRP2; PLRP2
Applications
Western Blot
Synonyms
PLRP2; Human PLRP2 HEK293 Overexpression Lysate; Human Galactolipase/PNLIPRP2 HEK293 Cell Lysate (WB positive control); pancreatic lipase-related protein 2; Human PLRP2 Overexpression Lysate; PNLIPRP2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
462aa
Sequence
Met1-Cys469
Applicable Applications for PNLIPRP2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human PNLIPRP2 (P54317) (Met 1-Cys469) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PNLIPRP2 cell lysate
Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. It can hydrolyze both phospholipids and galactolipids. Galactolipase acts preferentially on monoglycerides, phospholipids and galactolipids. It also hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase.
This Human PLRP2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PLRP2 protein from the overexpression lysate was verified.
This Human PLRP2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PLRP2 protein from the overexpression lysate was verified.
References
Andersson EL, et al. (2011) BSSL and PLRP2: key enzymes for lipid digestion in the newborn examined using the Caco-2 cell line. J Lipid Res. 52(11):1949-56.Xiao X, et al. (2011) Pancreatic lipase-related protein-2 (PLRP2) can contribute to dietary fat digestion in human newborns. J Biol Chem. 286(30):26353-63.Alves BN, et al. (2009) Lipid-dependent cytotoxicity by the lipase PLRP2 and by PLRP2-positive cytotoxic T lymphocytes (CTLs). Cell Biochem Funct. 27(5):296-308.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,947 Da
NCBI Official Full Name
pancreatic lipase-related protein 2
NCBI Official Synonym Full Names
pancreatic lipase-related protein 2
NCBI Official Symbol
PNLIPRP2
NCBI Official Synonym Symbols
PLRP2
NCBI Protein Information
pancreatic lipase-related protein 2; PL-RP2; galactolipase; OTTHUMP00000218565
UniProt Protein Name
Pancreatic lipase-related protein 2
Protein Family
UniProt Gene Name
PNLIPRP2
UniProt Synonym Gene Names
PLRP2
UniProt Entry Name
LIPR2_HUMAN
NCBI Description
This gene encodes a lipase that hydrolyzes galactolipids, the main components of plant membrane lipids. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the non-coding allele. [provided by RefSeq, Aug 2015]
Uniprot Description
PNLIPRP2: Lipase with broad substrate specificity. Can hydrolyze both phospholipids and galactolipids. Acts preferentially on monoglycerides, phospholipids and galactolipids. Contributes to milk fat hydrolysis. Belongs to the AB hydrolase superfamily. Lipase family.
Protein type: Lipid Metabolism - glycerolipid; Secreted; EC 3.1.1.26; Lipase; Secreted, signal peptide; EC 3.1.1.3
Chromosomal Location of Human Ortholog: 10q25.3
Cellular Component: extracellular space; extracellular region
Molecular Function: triacylglycerol lipase activity; galactolipase activity; acylglycerol lipase activity; calcium ion binding; phospholipase activity
Biological Process: triacylglycerol metabolic process; phospholipid catabolic process; galactolipid catabolic process; lipid digestion
Research Articles on PNLIPRP2
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Product Notes
The PNLIPRP2 pnliprp2 (Catalog #AAA8115244) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 462aa. AAA Biotech's PLRP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PNLIPRP2 pnliprp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Cys46 9. It is sometimes possible for the material contained within the vial of "PLRP2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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