PLRP2 Recombinant Protein | PNLIPRP2 recombinant protein

PLRP2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121509

• Gene Names: PNLIPRP2; PLRP2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PLRP2; PLRP2 Protein; Human; Recombinant (His Tag); Human Galactolipase/PNLIPRP2 Protein (His Tag); pancreatic lipase-related protein 2; PNLIPRP2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys469

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 18
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PNLIPRP2 (P54317)(Met 1-Cys469) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PNLIPRP2 recombinant protein

Background: Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. It can hydrolyze both phospholipids and galactolipids. Galactolipase acts preferentially on monoglycerides, phospholipids and galactolipids. It also hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase.

References

Andersson EL, et al. (2011) BSSL and PLRP2: key enzymes for lipid digestion in the newborn examined using the Caco-2 cell line. J Lipid Res. 52(11):1949-56.Xiao X, et al. (2011) Pancreatic lipase-related protein-2 (PLRP2) can contribute to dietary fat digestion in human newborns. J Biol Chem. 286(30):26353-63.Alves BN, et al. (2009) Lipid-dependent cytotoxicity by the lipase PLRP2 and by PLRP2-positive cytotoxic T lymphocytes (CTLs). Cell Biochem Funct. 27(5):296-308.

NCBI and Uniprot Product Information

• NCBI GI #: 106507261
• NCBI GeneID: 5408
• NCBI Accession #: NP_005387.2
• NCBI GenBank Nucleotide #: NM_005396.4
• UniProt Accession #: P54317; Q6IB55; A8K627
• Molecular Weight: 51,947 Da
• NCBI Official Full Name: pancreatic lipase-related protein 2
• NCBI Official Synonym Full Names: pancreatic lipase-related protein 2
• NCBI Official Symbol: PNLIPRP2
• NCBI Official Synonym Symbols: PLRP2
• NCBI Protein Information: pancreatic lipase-related protein 2; PL-RP2; galactolipase; OTTHUMP00000218565
• UniProt Protein Name: Pancreatic lipase-related protein 2
• Protein Family: Pancreatic lipase-related protein
• UniProt Gene Name: PNLIPRP2
• UniProt Synonym Gene Names: PLRP2
• UniProt Entry Name: LIPR2_HUMAN

NCBI Description

This gene encodes a lipase that hydrolyzes galactolipids, the main components of plant membrane lipids. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the non-coding allele. [provided by RefSeq, Aug 2015]

Uniprot Description

PNLIPRP2: Lipase with broad substrate specificity. Can hydrolyze both phospholipids and galactolipids. Acts preferentially on monoglycerides, phospholipids and galactolipids. Contributes to milk fat hydrolysis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Lipid Metabolism - glycerolipid; Secreted; EC 3.1.1.26; Lipase; Secreted, signal peptide; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: extracellular space; extracellular region

Molecular Function: triacylglycerol lipase activity; galactolipase activity; acylglycerol lipase activity; calcium ion binding; phospholipase activity

Biological Process: triacylglycerol metabolic process; phospholipid catabolic process; galactolipid catabolic process; lipid digestion