Lactoferrin/LTF Cell Lysate | LTF cell lysate
Mouse Lactoferrin/LTF HEK293 Overexpression Lysate
Gene Names
Ltf; Lf; Csp82; Ms10r; MMS10R
Applications
Western Blot
Synonyms
Lactoferrin/LTF; Mouse Lactoferrin/LTF HEK293 Overexpression Lysate; Mouse Lactotransferrin/LTF HEK293 Cell Lysate (WB positive control); lactotransferrin; Mouse Csp82 Overexpression Lysate; Mouse Lf Overexpression Lysate; Mouse MMS10R Overexpression Lysate; Mouse Ms10r Overexpression Lysate; LTF cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
699aa
Sequence
Met1-Gln707
Applicable Applications for LTF cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse LTF (AAH06904.1) (Met 1-Gln 707) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for LTF cell lysate
Lactotransferrin, also known as Lactoferrin, Talalactoferrin, and LTF, is a secreted protein that belongs to the transferrin family. Transferrins are iron binding transport proteins that can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B, and C have opioid antagonist activity. Lactoferrin A shows a preference for mu-receptors, while lactoferricin B and lactoferricin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin/LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin/LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin/LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin/LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucose. In particular, lactoferrin provides an antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.
This Mouse Lactoferrin/LTF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Lactoferrin/LTF protein from the overexpression lysate was verified.
This Mouse Lactoferrin/LTF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Lactoferrin/LTF protein from the overexpression lysate was verified.
References
Sanchez L, et al., 1992, Arch. Dis. Child. 67 (5): 657-61. Wakabayashi H, et al., 2000, J. Antimicrob. Chemother. 46 (4): 595-602. Nozaki A, et al., 2003, J. Biol. Chem. 278 (12): 10162-73. Azzam HS, et al., 2007, Liver Int. 27 (1): 17-25.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
77,838 Da
NCBI Official Full Name
Lactotransferrin
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
Ltf
NCBI Official Synonym Symbols
Lf; Csp82; Ms10r; MMS10R
NCBI Protein Information
lactotransferrin
Protein Family
Research Articles on LTF
Similar Products
Product Notes
The LTF (Catalog #AAA8116851) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 699aa. AAA Biotech's Lactoferrin/LTF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the LTF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gln70 7. It is sometimes possible for the material contained within the vial of "Lactoferrin/LTF, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
