Rabbit anti-Human Lactoferrin/LTF Polyclonal Antibody | anti-LTF antibody

Anti-Lactoferrin/LTF Antibody, Rabbit Polyclonal

Cat. Num. AAA8104867

• Gene Names: LTF; LF; HLF2; GIG12
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Lactoferrin/LTF; Polyclonal Antibody; Anti-Lactoferrin/LTF Antibody; Rabbit Polyclonal; Lactotransferrin/LTF Antibody; Rabbit PAb; Antigen Affinity Purified; lactotransferrin; Anti-GIG12 Antibody; Anti-HEL110 Antibody; Anti-HLF2 Antibody; Anti-LF Antibody; anti-LTF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Lactotransferrin/LTF
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LTF antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Human Lactotransferrin/LTF protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Lactotransferrin/LTF (rh Lactotransferrin/LTF). Lactotransferrin/LTF specific IgG was purified by Human Lactotransferrin/LTF affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human LTF in human spleen with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human LTF in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The image showing positive staining of leukocytes.)

Related Product Information for anti-LTF antibody

Lactotransferrin, also known as Lactoferrin, Talalactoferrin, and LTF, is a secreted protein that belongs to the transferrin family. Transferrins are iron binding transport proteins that can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B, and C have opioid antagonist activity. Lactoferrin A shows a preference for mu-receptors, while lactoferricin B and lactoferricin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin/LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin/LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin/LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin/LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucose. In particular, lactoferrin provides an antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.

References

Sanchez L, et al., 1992, Arch. Dis. Child. 67 (5): 657-61. Wakabayashi H, et al., 2000, J. Antimicrob. Chemother. 46 (4): 595-602. Nozaki A, et al., 2003, J. Biol. Chem. 278 (12): 10162-73. Azzam HS, et al., 2007, Liver Int. 27 (1): 17-25.

NCBI and Uniprot Product Information

• NCBI GI #: 2104522
• NCBI GeneID: 4057
• UniProt Accession #: P02788; O00756; Q16780; Q16785; Q16786; Q16789; Q5DSM0; Q8IU92; Q8IZH6; B2MV13; B7Z4X2; E7EQH5
• Molecular Weight: 78,182 Da
• NCBI Official Full Name: lactoferrin
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12
• NCBI Protein Information: lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN

NCBI Description

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.-; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; serine-type endopeptidase activity; iron ion binding

Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; regulation of cytokine production; activation of NF-kappaB transcription factor; retinal homeostasis; positive regulation of osteoblast differentiation; innate immune response in mucosa; negative regulation of viral genome replication; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of apoptosis